Mag-Bind® Blood DNA HV Kit

$0.00$2,040.10

Updated in 2024!

Isolate DNA from up to 4 mL whole blood using magnetic beads.

CE-IVD marked kit is available: Mag-Bind® Blood DNA HV Kit CE IVD – (omegabiotek.com)

M3292-00S Mag-Bind® Blood DNA HV Kit (Sample Request)

$0.00

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Blood DNA HV Kit is intended for isolation and purification of genomic DNA from up to 4 mL whole blood samples.
The Mag-Bind® Blood DNA HV Kit utilizes magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. The Mag-Bind® Blood DNA HV Kit can be used to process
up to 2 mL whole blood samples on Omega Bio-tek’s MagBinder® Fit24 Nucleic Acid Purification System when user-filled into MagBinder compatible cartridges.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting AmountUp to 4 mL
Starting MaterialWhole blood
Elution Volume0.4 – 1 mL
TechnologyMagnetic beads
Processing ModeAutomated, Manual
Throughput24
Note

Recovered DNA exhibits good purity with A260/A280 = ~1.8 and A260/A230 > 1.8.

High-quality DNA with excellent purity ratios

SampleDNA Yield (µg)A260/A280A260/A230
NanoDropQuantiFluor System
1119.21121.871.93
2120.11081.862.12
3114.91141.861.98
Table 1. DNA was manually extracted from 4 mL whole blood samples and was eluted in 1 mL volume. Purified DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system and Promega’s QuantiFluor® dsDNA system. The concordant readings indicate the DNA purified is primarily dsDNA.

qPCR Analysis of Purified DNA

Figure 1. Average Ct values obtained using human-specific primers on 10X and 100X dilutions of purified DNA used as the template. The Ct values differed by ~ 3 cycles per 10-fold dilution indicating inhibitor-free DNA.

DNA Yield from 2 mL whole blood samples purified on MagBinder Fit24 vs Manual

Figure 2. Average DNA Yield from 2 mL sample volume from three different lots of blood extracted manually vs automated on MagBinder® Fit24 Nucleic Acid Purification System. The DNA yield was determined using Thermo Scientific’s NanoDrop 2000c and found to be comparable between the manual and automated DNA extractions.

High Molecular Weight DNA extracted from blood samples

Figure 3. TapeStation® Analysis of Purified DNA. TapeStation® analysis of genomic DNA purified from 2 mL of whole blood samples using Omega Bio-tek’s Mag-Bind® Blood DNA HV Kit processed manually and on MagBinder® Fit24 Nucleic Acid Purification System.

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